Life is sustained through a delicate balancing act of the immune system, a complex network of molecular and cellular interactions from which health or disease can emerge. Despite a long catalogue of the cells and signaling proteins in this system, traditional experimental approaches have struggled to explain how they are organized in organs such as the lymph node to dynamically protect against infection, cancer, and autoimmunity. The overarching goal of my laboratory is to develop bioanalytical methods to visualize where, when, and how cells interact during immunity and inflammation, to i