Cells are poised to respond to their physical environment and to chemical stimuli in terms of collective molecular interactions that are regulated in time and space by the plasma membrane and its connections with the cytoskeleton and intracellular structures. Small molecules may engage specific receptors to initiate a transmembrane signal, and the surrounding system efficiently rearranges to amplify this nanoscale interaction to microscale assemblies, yielding a cellular response that often reaches to longer length scales within the organism.
Abstract: Access to global public healthcare is impacted by many technical, economic, and social factors. It is widely recognized that the resources required to deliver and improve global public health are currently constrained. A powerful way to increase access is to lower the cost of products and services that have already proven to be effective. Currently, the cost of producing a wide range of pharmaceutical products is higher than it needs to be.
This seminar will cover the recent advances in the design and fabrication of folding- and dynamics-based electrochemical biosensors. These devices, which are often termed electrochemical DNA (E-DNA), aptamer-based (E-AB), and peptide-based (E-PB) sensors, are fabricated via direct immobilization of a thiolated and methylene blue (MB)-modified oligonucleotide or peptide probe onto a gold electrode. Binding of an analyte to the probe changes its structure and/or flexibility, which, in turn, influences the electron transfer between the MB label and the interrogating electrode.
This talk will provide an overview of our group’s work using both standard and atypical high-performance computational chemistry modeling to elucidate atomic scale reaction mechanisms of catalytic reactions. I will introduce our toolkit of in silico methods for accurately modeling (electro)catalytic reactions in solvating environments. I will then present how in silico methods can be used for predictive insights into chemical and material design.
Antimicrobial, cytolytic, and cell-penetrating peptides, often called membrane-active peptides, belong to a variety of structural classes, including, alpha-helical, beta-sheet, unstructured, and cyclic polypeptides, among others. Those peptides were intensely studied in the 1990s and early 2000s with the hope of opening the door for urgently needed new antibiotics. For about 15 years we have studied the kinetics and thermodynamics of their interactions with lipid vesicles with the hope of understanding the mechanism of their function.