Antibody affinity reagents and reproducibility:
Professor Bhupal Ban | UVA - Antibody Engineering & Technology Core
Hosted by Professor Rebecca Pompano - *NOTE: (Dell 2 Room 100)
- Trends for isolation of monoclonal antibodies
- Current antibody reproducibility problem in academic institute
- Antibody validation: Standards, policies, and practices
- A new mindset for affinity application, evaluation, and authorization
- The advantages of recombinant antibody production methods over monoclonal and polyclonal antibody production methods.
- Phage display antibody production be adapted to produce the characteristic desired in applications such as multiple epitope recognition, tissues, and phenotype functional antibodies.
- Learn about protein chemistry and conjugation of antibody and drug
- Immobilize antibody onto different polymers, nanoparticles, liposome
Folding- and dynamics-based electrochemical biosensors
Professor Rebecca Lai | University of Nebraska-Lincoln
Hosted by Professor Jill Venton
This seminar will cover the recent advances in the design and fabrication of folding- and dynamics-based electrochemical biosensors. These devices, which are often termed electrochemical DNA (E-DNA), aptamer-based (E-AB), and peptide-based (E-PB) sensors, are fabricated via direct immobilization of a thiolated and methylene blue (MB)-modified oligonucleotide or peptide probe onto a gold electrode. Binding of an analyte to the probe changes its structure and/or flexibility, which, in turn, influences the electron transfer between the MB label and the interrogating electrode. These sensors are resistant to false positive signals arising from the non-specific adsorption of contaminants and perform well even when employed directly in whole blood, saliva, and other realistically complex sample matrices. Furthermore, because all of the sensing components are chemisorbed onto the electrode surface, they are readily regenerable and reusable. Our results show that many of these sensors have achieved state-of-the-art sensitivity while offering the unprecedented selectivity, reusability and operational convenience of direct electrochemical detection.