Upcoming Seminars

All seminars are held at 3:30 PM in the Mechanical Engineering Building (MEC) Room 205 unless otherwise noted.

Spring 2019

Jan
18

TBA

TBA

Professor Robbyn Anand | Iowa State University

Nathan Swami
Professor Robbyn Anand | Iowa State University
Hosted by Nathan Swami
Jan
25

TBA

TBA

Professor Shannon Stahl | University of Wisconsin-Madison

Professor Charlie Machan
Professor Shannon Stahl | University of Wisconsin-Madison
Hosted by Professor Charlie Machan
Feb
01

Screening, Isolation, and Characterization of Antibiotic Natural Products

Screening, Isolation, and Characterization of Antibiotic Natural Products

Professor Amanda Wolfe | University of North Carolina Asheville

Professor Mike Hilinski

Abstract

The increased emergence of bacterial resistance over the past two decades has greatly reduced the effectiveness of nearly all clinical antibiotics, bringing infectious disease to the forefront as a dire threat to global health. To combat these infections, new antibiotics need to be rapidly discovered, and bacterial natural products have reemerged as an abundant source of novel bioactive molecules. Herein, the isolation and evaluation of over 400 bacteria from bulk and rhizosphere soil native to western North Carolina and the southwestern U.S. in a novel and robust liquid-based high-throughput antagonism assay against Staphylococcus aureus and Escherichia coli is presented. Over 300 bacterial species were screened in monoculture, and 12% and 15% were found to produce antibiotics capable of ≥30% growth inhibition of Staphylococcus aureus or Escherichia coli respectively. 69 of those bacteria were subjected to 16s rRNA sequencing and found to be majority Pseudomonas (30%) and Serratia (17%) bacteria, and Aquitalea, Brevundimonas, Chryseobacterium, Herbaspirillum, and Microbacterium bacteria, which are currently not known to be antibiotic producers. More than 10 producing bacteria have been subjected to large scale culture and extraction techniques to isolate the produced antibiotic. One of those, a Pseudomonas sp., was found to produce the natural product pseudopyronine B, and we have further improved the antibiotic activity of this natural product through SAR evaluation of the alkyl side chains.

Professor Amanda Wolfe | University of North Carolina Asheville
Hosted by Professor Mike Hilinski
Feb
15

TBA

TBA

Dr. Rick Olson | Bristol-Myers Squibb

Professor Ken Hsu
Dr. Rick Olson | Bristol-Myers Squibb
Hosted by Professor Ken Hsu
Feb
22

TBD

TBD

Professor Matt Lockett | UNC Chapel Hill

Professor Rebecca Pompano
Professor Matt Lockett | UNC Chapel Hill
Hosted by Professor Rebecca Pompano
Mar
29

Hecht Lecture - TBA

Hecht Lecture - TBA

Professor Dale Boger | Scripps Research Institute; San Diego, CA

Professor Sid Hecht
Professor Dale Boger | Scripps Research Institute; San Diego, CA
Hosted by Professor Sid Hecht
Apr
05

TBD

TBD

Dr. Glen Alliger | ExxonMobil

Professor Charlie Machan
Dr. Glen Alliger | ExxonMobil
Hosted by Professor Charlie Machan
Apr
12

ACS Poster Session

ACS Poster Session

Apr
19

TBA

TBA

Professor Héctor D. Abruña | Cornell University

Professor Sen Zhang
Professor Héctor D. Abruña | Cornell University
Hosted by Professor Sen Zhang
Apr
26

TBA

TBA

Professor Jill Millstone | University of Pittsburgh

Professor Charlie Machan
Professor Jill Millstone | University of Pittsburgh
Hosted by Professor Charlie Machan